HPTLC separation of diastereomeric di- and tripeptides
Summary
Type: TLC Application #: 401080
Detection: spray with 1% ninhydrin in pyridine/ glacial acetic acid (5:1, v/v) and heat 5 min to 100 °C Conditions: Eluent: 1. 1 M acetic acid + 1 M hydrochloric acid in water / methanol (20%)
2. 0.5 M sodium acetate in water / methanol (20%) Authors: Lepri L Source: J Chromatogr 265 (1983) 328
Details
Legend: A) TLC impregnated with 4% N-dodecylpyridinum chloride (N-DPC) using eluent 2
1. L-Leu-L-Tyr,
2. D-Leu-L-Tyr,
3. L-Tyr-L-Arg,
4. L-Tyr-D-Arg,
m1 = mixture of 1 and 2,
m2 = mixture of 3 and 4.